929 Comprehensive proteomic identification and quantification of skin microbiota and serum extracellular vesicles in atopic dermatitis

Extracellular vesicles (EVs) have been found to be secreted by various cells and work as mediator communicate between transport of cellular components. Previously, we serum EVs (sEVs) contained Helicobacter pylori-derived peptide associated with an immune recognition resulting skin manifestation. Recent study proposed microbial alteration dysbiosis contribute atopic dermatitis (AD) pathogenesis. Therefore, analyzed microorganisms in sEVs assess the differences from microenvironment AD. In addition, recent microbiome analysis has mainly done metagenomics, however, current only estimates proportions taxa based on relative read counts. For this background, also aimed analyze proteomic profile quantify bacterial peptides. We collected samples AD non-AD groups for mass spectrometry. Metagenomic was used components samples. incubated or CD4+Tcells. Then, T cell differentiation flow cytometry. identified 6 species including Staphylococcus aureus both groups. Notably, that number peptides derived twice higher than non-AD. Moreover, percentage IL-4-producing CD4+T increased when treated compared those The result showed our protocol enabled find composition sEVs. containing bacteria related helper 2 differentiation. Herein, report first observations comprehensive quantitative relationships human microbiota. suppose influence immunity patients.